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viability assay kit  (Dojindo Labs)


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    Structured Review

    Dojindo Labs viability assay kit
    Viability Assay Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 95/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microbial+viability+assay+kit+wst/Microbial+Viability+Assay+Kit-WST/pm42093137-60-3-9
    Average 95 stars, based on 121 article reviews
    viability assay kit - by Bioz Stars, 2026-10
    95/100 stars

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    Viability Assay:

    Article Title: Development of an Alternative to Preservation Efficacy Tests Using the Metabolic Activity of Microorganisms.
    Article Snippet: .. Consequently, we chose to use a commercially available reagent, the Microbial Viability Assay Kit-WST (Dojindo Laboratories, Inc., hereafter referred to as WST-8), that colorimetrically detects the metabolic activity of microorganisms using the reducing ability of NAD[P]H. ..

    Article Title: A short peptide derived from late embryogenesis abundant proteins enhances acid tolerance in Escherichia coli via modulation of two‐component regulatory systems
    Article Snippet: .. Additionally, bacterial viability was assessed using the Microbial Viability Assay Kit‐WST (Dojindo, Kumamoto, Japan), following the manufacturer's instructions. .. Briefly, after the acid stress treatment for 2 h and resuspension in PBS, 190 μL of each culture was transferred to a 96‐well clear flat‐bottomed plate (Nunclon, Thermo Fisher Scientific), followed by the addition of 10 μL of WST reagent (prepared as a 9 : 1 mixture with DMSO).

    Article Title: A short peptide derived from late embryogenesis abundant proteins enhances acid tolerance in Escherichia coli via modulation of two-component regulatory systems.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense viability was assessed using the Microbial Viability Assay Kit-WST (Dojindo, Kumamoto, Japan), following the manufacturer’s instructions. .. Briefly, after the acid stress treatment for 2 h and resuspension in PBS, 190 μL of each culture was transferred to a 96-well clear flat-bottomed plate (Nunclon, Thermo Fisher Scientific), followed by the addition of 10 μL of WST reagent (prepared as a 9 : 1 mixture with DMSO).

    Article Title: Anti-Biofilm Activity of Combinations of Cinnamic Acid and Its Derivatives with Cloxacillin Against Methicillin-Resistant Staphylococcus epidermidis
    Article Snippet: .. After incubation, the mass of the formed biofilm was evaluated using a modified Christensen method [ ], and cell viability was assessed using the Microbial Viability Assay Kit-WST according to the manufacturer’s instructions (Dojindo Laboratories, Kumamoto, Japan). ..

    Article Title: Culture-Free Microfluidics for Ultra-Rapid Antimicrobial Susceptibility Testing with AI in Resource-Limited Settings.
    Article Snippet: The misuse of antibiotics has accelerated the rise of antimicrobial resistance (AMR), particularly in resource-limited regions, posing a critical threat to global public health.. Current antibiotic susceptibility test (AST) technologies are trapped in a paradox: regions most in need of rapid diagnostics face the greatest implementation barriers due to culture-based delays and infrastructure dependence.. No existing platform simultaneously overcomes these spatiotemporal constraints while maintaining phenotypic reliability.

    Article Title: Anti-Biofilm Activity of Combinations of Cinnamic Acid and Its Derivatives with Cloxacillin Against Methicillin-Resistant Staphylococcus epidermidis
    Article Snippet: .. After incubation at a temperature of 37 ± 1 °C for a period of 16 ± 2 h under aerobic conditions, the culture medium was removed, and viability was determined using the Microbial Viability Assay Kit-WST according to the manufacturer’s instructions (Dojindo Laboratories, Kumamoto, Japan) [ ]. .. The analysis was performed using a Multiskan EX Microplate Reader (Thermo Electron Corp., Vantaa, Finland).

    Article Title: Effective treatment of systemic candidiasis by synergistic targeting of cell wall synthesis.
    Article Snippet: .. The antifungal effect by FTNx was assessed using the Microbial Viability Assay Kit-WST (Dojindo Molecular Technologies, M439-10), according to the manufacturer’s instructions. .. Briefly, cultured fungal cells were washed with PBS and treated at 105 CFU/ml with FTNx (25 ~ 200 nM) or control formulations naked fso, or bare gold nanoparticles for 0 ~ 24h, followed by exchange with fresh media with or without micafungin (1 ~ 8μg/ml) and further incubation for 6 ~ 24 h. TheWST reagentwas then added, incubated at 37 °C for 1 ~ 2 h, and the absorbance was measured at 450nm using Infinite® 200 PRO (Tecan, Ltd.).

    Article Title: Microencapsulation in alginate-pectin based capsules of probiotic Lactobacillus plantarum WCFS1: pectin source and degree of blockwise distribution of methyl esters are critical parameters for the efficacy of protection under gastrointestinal conditions
    Article Snippet: .. In this experiment, the Microbial Viability Assay Kit-WST (WST-8, Dojindo, USA) was applied to quantify microbial metabolism. ..

    Activity Assay:

    Article Title: Development of an Alternative to Preservation Efficacy Tests Using the Metabolic Activity of Microorganisms.
    Article Snippet: .. Consequently, we chose to use a commercially available reagent, the Microbial Viability Assay Kit-WST (Dojindo Laboratories, Inc., hereafter referred to as WST-8), that colorimetrically detects the metabolic activity of microorganisms using the reducing ability of NAD[P]H. ..

    Article Title: Culture-Free Microfluidics for Ultra-Rapid Antimicrobial Susceptibility Testing with AI in Resource-Limited Settings.
    Article Snippet: The misuse of antibiotics has accelerated the rise of antimicrobial resistance (AMR), particularly in resource-limited regions, posing a critical threat to global public health.. Current antibiotic susceptibility test (AST) technologies are trapped in a paradox: regions most in need of rapid diagnostics face the greatest implementation barriers due to culture-based delays and infrastructure dependence.. No existing platform simultaneously overcomes these spatiotemporal constraints while maintaining phenotypic reliability.

    Incubation:

    Article Title: Anti-Biofilm Activity of Combinations of Cinnamic Acid and Its Derivatives with Cloxacillin Against Methicillin-Resistant Staphylococcus epidermidis
    Article Snippet: .. After incubation, the mass of the formed biofilm was evaluated using a modified Christensen method [ ], and cell viability was assessed using the Microbial Viability Assay Kit-WST according to the manufacturer’s instructions (Dojindo Laboratories, Kumamoto, Japan). ..

    Article Title: Anti-Biofilm Activity of Combinations of Cinnamic Acid and Its Derivatives with Cloxacillin Against Methicillin-Resistant Staphylococcus epidermidis
    Article Snippet: .. After incubation at a temperature of 37 ± 1 °C for a period of 16 ± 2 h under aerobic conditions, the culture medium was removed, and viability was determined using the Microbial Viability Assay Kit-WST according to the manufacturer’s instructions (Dojindo Laboratories, Kumamoto, Japan) [ ]. .. The analysis was performed using a Multiskan EX Microplate Reader (Thermo Electron Corp., Vantaa, Finland).

    Modification:

    Article Title: Anti-Biofilm Activity of Combinations of Cinnamic Acid and Its Derivatives with Cloxacillin Against Methicillin-Resistant Staphylococcus epidermidis
    Article Snippet: .. After incubation, the mass of the formed biofilm was evaluated using a modified Christensen method [ ], and cell viability was assessed using the Microbial Viability Assay Kit-WST according to the manufacturer’s instructions (Dojindo Laboratories, Kumamoto, Japan). ..



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